Review



p gp transporter inhibitor psc833  (Tocris)


Bioz Verified Symbol Tocris is a verified supplier
Bioz Manufacturer Symbol Tocris manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Tocris p gp transporter inhibitor psc833
    Fig. 1. Generation of stable human kidney cell lines with proven origin. A: Schematic of the human kidney and the cortex illustrates the source of isolated cells. B: Immunofluorescence staining of PDGFRβ+, CD10+ and CD31+ specific antibodies for cells in cortex of human kidney. C: Enumeration of the workflow from isolating the cells to the generation of genetically tagged cells. D: Representative pictures of genetically labeled immortalized PDGFRβ+, proximal tubular epithelial and endothelial cell lines. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. E: Principal component analysis (PCA) plot of the generated three cell lines. The three cell lines show higher variability from each other, while technical replicates are similar to each other. F: Shared DE genes between isolated cell lines and human bulk RNA seq [13]. G: CD10+ (red) cells stained for cell-specific proximal tubule epithelial markers cubilin (green, left) and CD13 (green, right) including DAPI. H: Heatmap of bulk RNA-sequencing data demonstrating the genes expressed in CD10+. I: P-gp Calcein-AM transporter assay of CD10+ and negative control PDGFRβ+ cells, with or without transporter inhibitor <t>PSC833.</t> ***p < 0.001 (one-way ANOVA analysis followed by Tukey post-test), n = 3 with each 12 replicates. J: Presence of intracellular vWF (red) in CD31+ (green) cells including DAPI. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    P Gp Transporter Inhibitor Psc833, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+gp+transporter+inhibitor+psc833/PSC+833/pm39705928-397-9-13
    Average 93 stars, based on 50 article reviews
    p gp transporter inhibitor psc833 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis."

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.

    Journal: Biomaterials

    doi: 10.1016/j.biomaterials.2024.123009

    Fig. 1. Generation of stable human kidney cell lines with proven origin. A: Schematic of the human kidney and the cortex illustrates the source of isolated cells. B: Immunofluorescence staining of PDGFRβ+, CD10+ and CD31+ specific antibodies for cells in cortex of human kidney. C: Enumeration of the workflow from isolating the cells to the generation of genetically tagged cells. D: Representative pictures of genetically labeled immortalized PDGFRβ+, proximal tubular epithelial and endothelial cell lines. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. E: Principal component analysis (PCA) plot of the generated three cell lines. The three cell lines show higher variability from each other, while technical replicates are similar to each other. F: Shared DE genes between isolated cell lines and human bulk RNA seq [13]. G: CD10+ (red) cells stained for cell-specific proximal tubule epithelial markers cubilin (green, left) and CD13 (green, right) including DAPI. H: Heatmap of bulk RNA-sequencing data demonstrating the genes expressed in CD10+. I: P-gp Calcein-AM transporter assay of CD10+ and negative control PDGFRβ+ cells, with or without transporter inhibitor PSC833. ***p < 0.001 (one-way ANOVA analysis followed by Tukey post-test), n = 3 with each 12 replicates. J: Presence of intracellular vWF (red) in CD31+ (green) cells including DAPI. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Fig. 1. Generation of stable human kidney cell lines with proven origin. A: Schematic of the human kidney and the cortex illustrates the source of isolated cells. B: Immunofluorescence staining of PDGFRβ+, CD10+ and CD31+ specific antibodies for cells in cortex of human kidney. C: Enumeration of the workflow from isolating the cells to the generation of genetically tagged cells. D: Representative pictures of genetically labeled immortalized PDGFRβ+, proximal tubular epithelial and endothelial cell lines. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. E: Principal component analysis (PCA) plot of the generated three cell lines. The three cell lines show higher variability from each other, while technical replicates are similar to each other. F: Shared DE genes between isolated cell lines and human bulk RNA seq [13]. G: CD10+ (red) cells stained for cell-specific proximal tubule epithelial markers cubilin (green, left) and CD13 (green, right) including DAPI. H: Heatmap of bulk RNA-sequencing data demonstrating the genes expressed in CD10+. I: P-gp Calcein-AM transporter assay of CD10+ and negative control PDGFRβ+ cells, with or without transporter inhibitor PSC833. ***p < 0.001 (one-way ANOVA analysis followed by Tukey post-test), n = 3 with each 12 replicates. J: Presence of intracellular vWF (red) in CD31+ (green) cells including DAPI. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Isolation, Immunofluorescence, Staining, Labeling, Generated, RNA Sequencing, Negative Control

    Related Articles

    Isolation:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).. Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.. After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.

    Immunofluorescence:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).. Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.. After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.

    Staining:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).. Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.. After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.

    Labeling:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).. Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.. After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.

    Generated:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).. Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.. After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.

    RNA Sequencing:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).. Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.. After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.

    Negative Control:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).When the cells reached 95 % confluence after 2–3 days, cells were washed with Krebs Henseleit (KH) (Merck, K3753) buffer (PH 7.4).. Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.. After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.After incubation, cells were washed 2 times with KH buffer before incubating for 45–60 min in 1 % Triton in KH buffer.



    Similar Products

    p gp transporter inhibitor psc833  (Tocris)
    93
    Tocris p gp transporter inhibitor psc833
    Fig. 1. Generation of stable human kidney cell lines with proven origin. A: Schematic of the human kidney and the cortex illustrates the source of isolated cells. B: Immunofluorescence staining of PDGFRβ+, CD10+ and CD31+ specific antibodies for cells in cortex of human kidney. C: Enumeration of the workflow from isolating the cells to the generation of genetically tagged cells. D: Representative pictures of genetically labeled immortalized PDGFRβ+, proximal tubular epithelial and endothelial cell lines. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. E: Principal component analysis (PCA) plot of the generated three cell lines. The three cell lines show higher variability from each other, while technical replicates are similar to each other. F: Shared DE genes between isolated cell lines and human bulk RNA seq [13]. G: CD10+ (red) cells stained for cell-specific proximal tubule epithelial markers cubilin (green, left) and CD13 (green, right) including DAPI. H: Heatmap of bulk RNA-sequencing data demonstrating the genes expressed in CD10+. I: P-gp Calcein-AM transporter assay of CD10+ and negative control PDGFRβ+ cells, with or without transporter inhibitor <t>PSC833.</t> ***p < 0.001 (one-way ANOVA analysis followed by Tukey post-test), n = 3 with each 12 replicates. J: Presence of intracellular vWF (red) in CD31+ (green) cells including DAPI. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    P Gp Transporter Inhibitor Psc833, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+gp+transporter+inhibitor+psc833/PSC+833/pm39705928-397-9-13
    Average 93 stars, based on 1 article reviews
    p gp transporter inhibitor psc833 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1. Generation of stable human kidney cell lines with proven origin. A: Schematic of the human kidney and the cortex illustrates the source of isolated cells. B: Immunofluorescence staining of PDGFRβ+, CD10+ and CD31+ specific antibodies for cells in cortex of human kidney. C: Enumeration of the workflow from isolating the cells to the generation of genetically tagged cells. D: Representative pictures of genetically labeled immortalized PDGFRβ+, proximal tubular epithelial and endothelial cell lines. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. E: Principal component analysis (PCA) plot of the generated three cell lines. The three cell lines show higher variability from each other, while technical replicates are similar to each other. F: Shared DE genes between isolated cell lines and human bulk RNA seq [13]. G: CD10+ (red) cells stained for cell-specific proximal tubule epithelial markers cubilin (green, left) and CD13 (green, right) including DAPI. H: Heatmap of bulk RNA-sequencing data demonstrating the genes expressed in CD10+. I: P-gp Calcein-AM transporter assay of CD10+ and negative control PDGFRβ+ cells, with or without transporter inhibitor PSC833. ***p < 0.001 (one-way ANOVA analysis followed by Tukey post-test), n = 3 with each 12 replicates. J: Presence of intracellular vWF (red) in CD31+ (green) cells including DAPI. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biomaterials

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.

    doi: 10.1016/j.biomaterials.2024.123009

    Figure Lengend Snippet: Fig. 1. Generation of stable human kidney cell lines with proven origin. A: Schematic of the human kidney and the cortex illustrates the source of isolated cells. B: Immunofluorescence staining of PDGFRβ+, CD10+ and CD31+ specific antibodies for cells in cortex of human kidney. C: Enumeration of the workflow from isolating the cells to the generation of genetically tagged cells. D: Representative pictures of genetically labeled immortalized PDGFRβ+, proximal tubular epithelial and endothelial cell lines. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. E: Principal component analysis (PCA) plot of the generated three cell lines. The three cell lines show higher variability from each other, while technical replicates are similar to each other. F: Shared DE genes between isolated cell lines and human bulk RNA seq [13]. G: CD10+ (red) cells stained for cell-specific proximal tubule epithelial markers cubilin (green, left) and CD13 (green, right) including DAPI. H: Heatmap of bulk RNA-sequencing data demonstrating the genes expressed in CD10+. I: P-gp Calcein-AM transporter assay of CD10+ and negative control PDGFRβ+ cells, with or without transporter inhibitor PSC833. ***p < 0.001 (one-way ANOVA analysis followed by Tukey post-test), n = 3 with each 12 replicates. J: Presence of intracellular vWF (red) in CD31+ (green) cells including DAPI. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Cells were treated for 1 h with 5 μM P-gp transporter inhibitor PSC833 (Tocris Biosciences, Bristol, UK) in KH buffer at 37 ◦C, 5 % (v/v) CO2, and afterwards exposed for 1 h to 1 μM Calcein-AM (Life Technologies Europe BV) with or without 5 μM PSC833 in KH buffer.

    Techniques: Isolation, Immunofluorescence, Staining, Labeling, Generated, RNA Sequencing, Negative Control